| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 47(2), 213-218, 2001
In Vivo Testing System for Determining the Estrogenic Activity of Endocrine-Disrupting Chemicals (EDCs) in Goldfish (Carassius auratus)
Hiroshi Ishibashi,a Katsuyasu Tachibana,a Mutsuyosi Tsuchimoto,a Kiyoshi Soyano,a Yasuhiro Ishibashi,b Masaki Nagae,c
Shinya Kohra,c Yuji Takao,c
Nobuaki Tominaga,d and Koji Arizono*, e
aGraduate School of Science and Technology, bEnvironmental Protection center, and cFaculty of Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan, dDepartment of Chemical and Biological Engineering, Ariake National College of Technology, 150 Higashihagio-machi, Omuta 836-8585, Japan, and eFaculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Kumamoto 862-8502, Japan
Vitellogenin (VTG) is believed to be an effective and sensitive biomarker to detect the effects of endocrine-disrupting chemicals (EDCs) on fish. An enzyme-linked immunosorbent assay (ELISA) was developed in this study for the quantification of VTG in goldfish (Carassius auratus) blood plasma using a monoclonal antibody against carp lipovitellin which is known to cross-react with goldfish VTG, and a working range of 7.8 to 500 ng VTG/ml was established. A laboratory study involving the dosing of male goldfish for 28 days with a range of bisphenol-A (BPA) concentrations was conducted, and the established ELISA was used. There was no significant induction of VTG at the concentrations of 1 and 10 mu g/l. Exposure to 100 mu g/l and 1000 mu g/l BPA for 28 days significantly elevated plasma VTG concentrations to 201 ± 90 mu g/ml and 104552 ± 24920 mu g/ml, respectively. To confirm the effect of temperature on VTG induction, male goldfish were exposed to the nominal concentration of 17beta-estradiol (E2) 100 mu g/l at 10° and 30°C for 10 days. Plasma VTG concentration in male goldfish increased more quickly in the fish exposed at 30°C than in those at 10°C. In addition, the plasma VTG concentration in the 30°C group (10463 ± 3268 mu g/ml) was higher than that of the 10°C group (1.42 ± 1.6 mu g/ml) after 1 day of exposure. Thus goldfish are useful for the investigation of EDCs under various conditions since they are relatively small in size, and the ELISA established in the present study will contribute to various types of research.
|
|
