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J.Health Sci., 46(6), 493-502, 2000
A New Assay for Evaluating Hepatotoxicity and Cytotoxicity Using LDL-Uptake Activity of Liver Cells
Ryo Shoji,*, a Akiyoshi Sakoda,b
Yasuyuki Sakai,b Hideo Utsumi,c and Motoyuki Suzuki b,d
aDepartment of Chemical Science and Engineering, Tokyo National College of Technology, 1220-2 Kunugida-machi, Hachioji-shi, Tokyo 193-0997, Japan, bInstitute of Industrial Science, University of Tokyo, 7-22-1 Roppongi, Minato-ku, Tokyo 106-8558, Japan, cFaculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, and dThe United Nations University, 52-70-5 Jingumae, Shibuya-ku, Tokyo 150-8925, Japan
A rapid and sensitive bioassay for detecting cytotoxicity was developed in this study to be used in evaluating many kinds of chemicals. This assay, based on the LDL (low density lipoprotein)-uptake activity of human hepatoblastoma cells, Hep G2, can evaluate cytotoxicity for 48 h with high sensitivity and selectivity using a 96 well plate and a fluorescent plate reader. We evaluate the toxicity of 230 kinds of chemicals and formulate the dose response data by a simple mathematical equation. The toxicity parameters derived by the formulation had some correlations in terms of chemical groups, which were classified as aromatics, organics, metals, and so on.
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