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J.Health Sci., 46(5), 351-357, 2000
Simple Pretreatment Method for Monitoring of Norephedrine in Urine and Tyramine in Food Sample Using Liquid-Liquid Extraction
Hideo Isono,* Toshihide Suzuki, and Hisao Nojiri
Faculty of Pharmaceutical Sciences, Teikyo University, 1091 1 Suwarashi, Sagamiko-machi, Tsukui-gun, Kanagawa 199 0195, Japan
Norephedrine and tyramine are familiar as sympathetic nerve-stimulating chemicals.
Analysis of norephedrine in urine sample is necessary for the evaluation of intake.
Analysis of tyramine in food samples is also necessary for evaluation of its daily intake. However, both are difficult to extract by conventional liquid liquid extraction.
The present work proposed to monitor both using a small amount of a mixture of two solvents. For norephedrine,
a filtrate (100 ml) of human urine sample (pH 11) was mixed with 5% sodium dodecyl sulfate solution (100 mu l) and acetone (2.5 ml),
then shaken with chloroform (2.5 ml).
This method recovered 5-10% of norephedrine added. For tyramine, a filtrate (5 ml) of intact or concentrated sample solution (pH 11) was salted out with NaCl (1 g),
then shaken with a mixture of acetone (3 ml)
and ethyl ether (2 ml). This method recovered 50-60% of tyramine added.
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