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J.Health Sci., 46(5), 343-350, 2000
Cyanide and Thiocyanate Levels in Blood and Saliva of Healthy Adult Volunteers
Kouichiro Tsuge, Mieko Kataoka, and Yasuo Seto*
National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan
Cyanide and thiocyanate levels were determined by head space gas chromatography and the spectrophotometric Konig method respectively,
for blood and salivary samples collected from 40 healthy adult volunteers. Blood cyanide (mean plus/minus standard deviation: 0.27 plus/minus 0.07 mu M), plasma thiocyanate
(111.2 plus/minus 92.1 mu M), salivary cyanide (0.66 plus/minus 0.52 mu M) and salivary thiocyanate (1655 plus/minus 841 mu M) levels were significantly higher in the group of 20 tobacco smokers than in the group of 20 nonsmokers (0.17 plus/minus 0.04 mu M, 33.5 plus/minus 25.4 mu M, 0.38 plus/minus 0.26 mu M,
542 plus/minus 406 mu M).
Statistical correlations were observed between the blood cyanide and the salivary thiocyanate levels (gamma = 0.64), between the blood cyanide and the plasma thiocyanate levels (gamma = 0.54),
and between the plasma thiocyanate and the salivary thiocyanate levels (gamma = 0.46). It is concluded that not only plasma and salivary thiocyanate levels but also blood cyanide level can be suitable indices for distinguishing smokers from nonsmokers.
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