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J.Health Sci., 46(4), 310-313, 2000
Cross-Reactivity of Various Tetrahydrocannabinol Metabolites with a Monoclonal Antibody against Tetrahydrocannabinolic Acid
Kazuhito Watanabe,a Midori Matsuda,a Yuji Tateoka,a Toshiyuki Kimura,a Tamihide Matsunaga,a Hiroyuki Tanaka,b Yukihiro Shoyama,b and Ikuo Yamamoto*,a
aDepartment of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa 920-1181, Japan and bDepartment of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
A monoclonal antibody (MAb-4A4) against Delta9-tetrahydrocannabinolic acid (THCA) was prepared and its cross-reactivity analyzed with various cannabinoids including tetrahydrocannabinol metabolites by competitive enzyme-linked immunoassay (ELISA). Delta9-THC (1-100 mug/ml) could be determined by the ELISA. Many metabolites of Delta8-THC and Delta9-THC reacted with the antibody, and their cross-reactivities were 44-157% of Delta8-THC. However, the antibody did not recognize the lipophilic compounds cholesterol, testosterone, beta-carotene, androstene-3,17-dione or an endogenous cannabinoid, anandamide.
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