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J.Health Sci., 46(4), 259-262, 2000
Accumulation and Subcellular Localization of Cesium in Mycelia of Streptomyces lividans and a Cs Tolerant Strain, Streptomycessp. TOHO-2
Fumio Kato,*,a Chikako Kuwahara,b Ayako Oosone,a Takayuki Ichikawa,a Hiroshi Terada,c Yohoji Morita,d and Hideo Sugiyamac
aDepartment of Microbiology, School of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan, bDivision of Enviromental Health, Kanagawa Prefectural Public Health Laboratory, 1-1-1 Nakao, Asahi-ku, Yokohama 241-0851, Japan, cNational Institute of Public Health, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8638, Japan, and dApplication Center, Horiba Co., Ltd., 2, Miyanohigashi, Kisshoin, Minami-ku, Kyoto 601-8150, Japan
Cs uptake in Streptomyces lividans TK24 and Streptomyces sp. TOHO-2, as representatives of actinomycetes, was investigated. Growth of S. lividans TK24 was inhibited above 50 mM CsCl in yeast extract-malt extract agar, while that of Streptomyces sp. TOHO-2, which was isolated from soil as a Cs tolerant strain, was inhibited above 200 mM CsCl. The amount of Cs incorporated into the mycelia of these strains increased with the concentration of Cs in the medium; S. lividans TK24, grown in the presence of 25 mM Cs, accumulated 61.5 mgCs/g dry weight of mycelia, and Streptomyces sp. TOHO-2, grown in the presence of 150 mM Cs, accumulated 142.2 mgCs/g dry weight of mycelia. Scanning electron microscopy (SEM) of the substrate mycelia grown on the YM agar plate containing CsCl showed white spots with a similar distribution of mycelia. Elemental analysis using SEM-energy dispersive X-ray analysis showed that there were larger amounts of Cs, P and O in the brilliant spots than in other regions of the cell. Streptomyces lividans TK24 and Streptomyces sp. TOHO-2 could grow even in the presence of high concentrations of Cs, and accumulated high concentration of Cs in the cells.
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