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J.Health Sci., 46(1), 16-20, 2000
Determination of Methamphetamine in Human Urine by HPLC Using Post-Column Simon's Reaction
Satoshi Chinaka,*,a Seishi Tanaka,a Nariaki Takayama,a and Kazumasa Uedab
aForensic Science Laboratory, Ishikawa Prefectural Police Headquarters, 2-1-1 Hirosaka, Kanazawa 920-8553, Japan and bDepartment of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University, 2-40 Kodatsuno, Kanazawa 920-8667, Japan
An HPLC method using post-column Simon's reaction (SR-HPLC) for the determination of methamphetamine (MA) has been developed. The detection limit was 1 ng, which is about 100 times more sensitive than TLC using Simon's reaction (SR-TLC). The calibration curve was linear in the range of 0.1 -1000 mu g/ml, and the intermediate precision of within-run and between-run assays was 1.15% and 2.12%, respectively. MA in 13 urine samples was determined by SR-HPLC and GC with flame ionization detection, and these quantitative values were strongly correlated (r2=0.995). MA could be analyzed without interference peaks. The proposed method is highly selective and quantitative.
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