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J.Health Sci., 45(6), 384-390, 1999
Effect of 2-Hexenal on the Lipid Peroxidation of Primary Cultured Rat Hepatocytes[inJapanese]
Teruhisa Hirayama,*Akihiro Yoshikawa, Terue Kasai, and Tetsushi Watanabe
Kyoto Pharmaceutical University, 5-Nakauchi-cho, Misasagi, Yamashinaku, Kyoto 607-8414, Japan
Hepatocytes isolated from male Wister rats with or without pre-treatment of phenobarbital (PB), 3-methylcholanthrene (3-MC), beta-naphthoflavone (beta-NF) and dexamethasone (DMX) by the previous method were cultured in dishes in an atmosphere of 5% CO2 -95% air at 37 degrees C.
Rat hepatocytes in culture for 24 h were incubated in Williams'E medium containing 2-hexenal and tert-butylhydroperoxide.
Cell membrane oxidation was measured by the fluorometric thiobarbituric acid (TBA) method.
Cell membrane oxidation induced by 2-hexenal and tert-butylhydroperoxide and inhibited by the addition of some antioxdants, was only observed in whole hepatocytes treated with 2-hexenal, but not in the purified cell membrane under the same conditions.
Therefore, it is assumed that 2-hexenal converted to active substances could injure the membrane of hepatocytes.
In PB pretreated-rat hepatocytes, increasing TBA-RS formation was observed.
Finally, it is concluded that 2-hexenal is activated in hepatocytes by CYP2B, and that the membrane lipid peroxidation is caused by active compounds such as radicals.
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