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J.Health Sci., 45(4), 222-225, 1999
Selective and Simple Quantification of Metallothionein III in Mouse Brain
Akira Yasutake,*,a Masahiko Satoh,b Chiharu Tohyamab and Kimiko Hirayamac
aBiochemistry Section, National Institute for Minamata Disease, Minamata, Kumamoto 867-0008, Japan, bEnvironmental Health Sciences Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-0053, Japan, and cKumamoto University, College of Medical Science, 2-24-1 Kuhonji, Kumamoto 862-0976, Japan
For the selective quantification of metallothionein (MT)-III, MT samples from mouse brain were converted to Hg-MT, then chromatographed using a double (connected-in-tandem) FPLC-gel permeation column system. The chromatogram showed two peaks with a slight overlap. Hg-MT-III was eluted prior to Hg-MT-I and II, probably due to a slightly higher molecular weight. The amount of each isomer was determined from an analysis of the Hg content in each fraction. The present procedure revealed that MT-III in the mouse brain was comparable in quantity to MT-I and II. Thus, MT-III in mouse brain could be selectively quantified at a sub-picomol level.
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